AIM: To simultaneously determinate the six major components in Semen Ziziphi Spinosae (Suanzaoren) by high performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) for a scientific evaluation of its quality. METHOD: The separation was achieved on a CLC-ODS C18 analytical column. The mobile phase consisted of 0.1% aqueous acetic acid (A) and acetonitrile (B) using a gradient elution. The drift tube temperature of ELSD was set at 106.5℃, and nitrogen flow-rate was 2.9 L•min^(-1). RESULT: Three flavonoids (isovitexin-2”-O-β-glucopyranoside, spinosin and 6'”-feruloylspinosin) and three saponins (jujuboside A, jujuboside B and jujuboside D) showed good linearity (r^2＞0.999) within test ranges. This method was well validated with good precision, repeatability and sensitivity. It was successfully applied to quantify three flavonoids and three saponin components in twenty-five samples. CONCLUSION: The developed simple, rapid and reliable HPLC-ELSD method is suitable for the comprehensive quality control of Suanzaoren as well as its preparations.
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Bao et al. (2009) studied this question.
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