Key result
In vivo analyses of turnip crinkle carmovirus suggest it uses a premature termination mechanism for subgenomic mRNA transcription.
Minus-strand sgRNA detection and uncoupling in carmovirus; leaves open upstream structure roles for mechanistic studies.
The transcriptional mechanism utilized by turnip crinkle carmovirus to synthesize subgenomic (sg) mRNAs was investigated by analyzing viral RNAs and their associated regulatory RNA elements. In vivo analyses revealed the following: (i) that minus-strand sg RNAs are detectable in infections, (ii) that minus-strand sg RNA accumulation can be partially uncoupled from that of their plus-strand sg mRNA counterparts, and (iii) that an RNA secondary structure located upstream of the sg mRNA start site mediates transcription by functioning in the plus strand of the viral genome. Collectively, these observations are consistent with this carmovirus using a premature termination mechanism for sg mRNA transcription.
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Wu et al. (2010) studied Turnip crinkle carmovirus infection. In vivo analyses of turnip crinkle carmovirus suggest it uses a premature termination mechanism for subgenomic mRNA transcription.
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