Key result
Laboratory diagnosis of nonpolio enteroviruses relies mainly on molecular techniques, with viral typing increasingly utilizing full-genome next-generation sequencing.
Why the study?
Nonpolio enterovirus infections are highly prevalent and cause substantial morbidity and mortality, making accurate laboratory diagnosis important for prognosis and clinical management guidance.
Full-genome next-generation sequencing is advancing the diagnosis of nonpolio enteroviruses by enabling comprehensive typing, outbreak investigation, and detection of severity markers.
Infections by nonpolio enteroviruses (EVs) are highly prevalent, particularly among children and neonates, where they may cause substantial morbidity and mortality. Laboratory diagnosis of these viral infections is important in patient prognosis and guidance of clinical management. Although the laboratory diagnosis of nonpolio EVs is mainly based on molecular techniques, classical virus-isolation techniques are still used in reference laboratories. Other techniques, such as antigen detection and serology, are becoming obsolete and rarely used in diagnosis. An important part of diagnosis and surveillance of EV infections is viral typing by VP1 gene sequencing using conventional Sanger technique and more recently, full-genome next-generation sequencing. The latter allows the typing of all EVs, better investigation of EV outbreaks, detection of coinfection, and identification of severity markers in the EV genome.
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Itani et al. (2022) conducted a review in Nonpolio enterovirus infections. Laboratory diagnosis techniques was evaluated. Laboratory diagnosis of nonpolio enteroviruses relies mainly on molecular techniques, with viral typing increasingly utilizing full-genome next-generation sequencing.
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