April 1, 2001Journal of Clinical MicrobiologyOpen Access

Type-Specific Detection of Echovirus 30 Isolates Using Degenerate Reverse Transcriptase PCR Primers

Q: What is the clinical evidence from this study?

Study design: Other. Population: Echovirus 30 infection (n=284). Intervention: Degenerate RT-PCR primers vs. Other enterovirus serotypes. Primary outcome: Specific RT-PCR amplification of a 158-bp product.

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Key result

Degenerate RT-PCR primers specifically amplified a 158-bp product in all 221 echovirus 30 strains tested, with no amplification observed in 63 other enterovirus serotypes.

Population

221 echovirus 30 strains isolated in 16 countries over a 44-year period, and 63 other enterovirus serotypes

Design

Preclinical

Authors

DKDavid R. KilpatrickSouthern Illinois University Carbondale JQJacqueline QuayCenters for Disease Control and Prevention MPMark A. PallanschNorthwestern University

Discussion

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Implication

Enables specific echovirus 30 detection in isolates; leaves open clinical validation for routine diagnostics.

Structured PICO

Population

221 echovirus 30 strains isolated in 16 countries over 44 years and 63 other enterovirus serotypes tested for specific RT-PCR amplification.

Intervention

Reverse transcriptase (RT) PCR primers containing mixed-base residues or deoxyinosine at positions of codon degeneracy targeting conserved amino acid motifs within the major capsid protein, VP1

Outcome

Specific RT-PCR amplification of echovirus 30 (E30) sequences (yielding predicted 158-bp PCR product)surrogate

Main Result

Absolute Event Rate: 100% vs 0%

Degenerate RT-PCR primers containing inosine can specifically and reliably identify echovirus 30 isolates.

Cite This Study

Kilpatrick et al. (2001) studied Echovirus 30 infection (n=284). Degenerate RT-PCR primers vs. Other enterovirus serotypes was evaluated on Specific RT-PCR amplification of a 158-bp product. Degenerate RT-PCR primers specifically amplified a 158-bp product in all 221 echovirus 30 strains tested, with no amplification observed in 63 other enterovirus serotypes.

synapsesocial.com/papers/6a5ecdc39e99e7407a7e2f4b https://doi.org/10.1128/jcm.39.4.1299-1302.2001

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Type-Specific Detection of Echovirus 30 Isolates Using Degenerate Reverse Transcriptase PCR Primers

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Type-Specific Detection of Echovirus 30 Isolates Using Degenerate Reverse Transcriptase PCR Primers

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider