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July 22, 2026PLoS ONE0 citationsOpen Access

Assessment of waterlogging tolerance in tea genotypes through morpho-physiological and biochemical profiling

MAMd. Riyadh Arefin SMShitosri Mondal MRMd. Fazle Rabbi

Key Points

This research aims to evaluate morpho-physiological and biochemical responses of tea genotypes under waterlogging stress to identify tolerant varieties.
Evaluated eight tea genotypes and two control clones using 23 parameters in a two-factorial randomized design.
One group underwent 14 days of waterlogging followed by 14 days of recovery, while a control group was maintained under normal conditions.
Stress tolerance indices were calculated based on measured parameters.
P/AFN/13/90 emerged as the most waterlogging-tolerant genotype with superior stress tolerance indices.
Significant decreases in root fresh weight, leaf number, and net photosynthesis were observed under stress conditions compared to control.
Increased proline content and total antioxidant activity were noted, suggesting adaptive biochemical responses.

Abstract

Tea is one of the major economic crops in Bangladesh, however, its productivity is severely constrained by waterlogging stress arising from erratic climatic changes. A significant knowledge gap persists regarding the morpho-physiological and biochemical determinants of waterlogging tolerance and post-stress recovery in tea, hindering the development of tolerant genotypes. Therefore, the present study aimed to investigate the integrated morpho-physiological and biochemical responses of tea genotypes under waterlogging stress to identify tolerant genotype(s) based on stress tolerance indices (STIs). Eight pre-screened tea genotypes—P/LAL/08/23, P/LAL/08/62, P/LAL/09/116, P/AFN/11/35, P/AFN/11/46, P/OTI/31, P/AFN/13/90, P/AFN/11/31, with two control clones, BT2 (popular variety) and TV9 (waterlogging-tolerant variety), were evaluated using 23 morpho-physiological and biochemical parameters maintaining a two-factorial completely randomized design experiment. One set of tea genotypes was exposed to a 14-day waterlogging phase, followed by a 14-day recovery phase (total 28 days of stress), while another set of plants was maintained under control (non-stress) conditions, for the same period, to calculate STIs. Root fresh weight (RFW), leaf number (NL), net photosynthesis ( Pn ), transpiration rate ( E ), stomatal conductance ( g s ), relative leaf water content (RWC), and absolute growth rate (AGR) were significantly decreased under waterlogging condition compared to the control. Leaf chlorophyll a (CHA), chlorophyll b (CHB), and total carotenoids (CRTN), were reduced under stress, whereas proline content (of leaf: PRLF and root: PRRT), total antioxidant activity (of leaf: TACL and root: TACR), and lipid peroxidation (of leaf: LOPL and root: LPOR) increased as a part of adaptive responses. The traits such as RFW, NL, Pn , E , g s , RWC, AGR, CHA, CHB, CRTN, and LPOL, were detected as most influential at both phases of stress conditions from principal component analysis. Ultimately, the P/AFN/13/90 was identified as the most waterlogging-tolerant genotype, exhibiting higher STIs for maximum traits during both the waterlogging and recovery phases.

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