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Complete genome sequencing reveals key genetic features of PCR ribotype 001 Clostridioides difficile strain NCTC 12727 highlighting its genetic complexity.
We report the complete genome sequence of the PCR ribotype 001 Clostridioides difficile strain NCTC 12727 obtained by hybrid genome assembly, manual curation, and PCR validation. The circular 4,371,963 bp genome (28.99% GC) contained two multi-copy mobile genetic elements encompassing a prophage and a Tn 916 -like element, respectively.
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Venkatesan et al. (2026) studied this question.
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