Effects of Testosterone on Analgesia in Formalin-Treated Mice

Background & objectives: So far, various reports have been presented on the relationship between sex hormones and gender-related differences in pain and analgesia in humans and laboratory animals. The purpose of this study was to investigate the effect of testosterone hormone and spironolactone anti-androgen drug on morphine-induced analgesia in male mice using formalin test. Methods: In this study, 80 male mice were divided into 10 groups (N=8); normal saline (control), sesame seed oil (as testosterone solvent), testosterone (5 and 10 mg/kg body weight), spironolactone, morphine, sesame seed oil + morphine, testosterone (5 and 10 mg/ kg body weight) + morphine and spironolactone + morphine. Formalin test was performed in all the mice, and data were analyzed by one-way ANOVA.