Preventive effect and mechanism of Tibetan tea extract on thrombosis in arachidonic acid-induced zebrafish determined via RNA-seq transcriptome profiles

Figures

• 

  Fig 1. The establishment of zebrafish thrombus model. Representative images (A) and quantification (D) of o-dianisidine staining at the heart regions of control, AA and aspirin groups. Representative images (B) and quantification (E) of o-dianisidine staining at the tail regions control, AA and aspirin, treated. (C) Blood cell aggregation of Tg(gata1:DsRed) zebrafish in control group, AA group, and aspirin group. (F) Blood flow velocity frequency diagram of zebrafish in control, AA, and aspirin. Compared with the control group; compared with the model group,* for P < 0.05, ** for P < 0.01, *** for P < 0.001.(Note: The length of the scale bar in the figure is 500 μm).

• 

  Fig 2. Screening of optimal treatment concentration of Tibetan tea. Illustration of the timeline of drug protection and AA treatment. (B) Phenotype diagram of the effect of different concentrations of TT on zebrafish. (C) Effects of different TT concentrations on the hatching rate of zebrafish. (D) Effects of Different TT Concentrations on Zebrafish Mortality. (Note: The length of the scale bar in the figure is 500 μm).

• 

  Fig 3. Effects of TT on thrombus model of zebrafish. Representative images (A) and quantification (D) of o-dianisidine staining at the heart regions of control, AA and TT groups. The heart regions are circled by red dotted line. Representative images (B) and quantification (E) of o-dianisidine staining at the tail regions control, AA,and TT treated. (C) Blood cell aggregation of Tg(gata1:DsRed) zebrafish in control group, AA group, and TT group. (F) Blood flow velocity frequency diagram of zebrafish in control, AA, and TT. (G) Effective rate of Tibetan tea rescue (%). Compared with the control group; compared with the model group,* for P < 0.05, ** for P < 0.01, *** for P < 0.001. (Note: The length of the scale bar in the figure is 500 μm).

• 

  Fig 4. Effects of TT on oxidative stress levels in zebrafish thrombosis models. Representative images (A) and quantification (B) of the fluorescence signals of DCFH-DA staining in different treatment groups. Concentration of MDA (C) and SOD (D) in zebrafish in different treatment groups. Compared with the control group; compared with the model group,*P < 0.05, **P < 0.01, ***P < 0.001. (Note: The length of the scale bar in the figure is 500 μm).

• 

  Fig 5. RNA-seq data analysis. (A) Venn diagram of gene counts expressed. (B) Significant DEG counts. (C) The KEGG pathway enrichment of DEGs between AA and control. (D) The KEGG pathway enrichment of DEGs between AA and AA _TT.

• 

  Fig 6. Changes of thrombus-related gene expression. Compared with the control group; compared with the model group,*P < 0.05, **P < 0.01, ***P < 0.001.