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This randomized trial demonstrates the utility of a novel cyanine dye as a pH sensor in biological media, suggesting its application in scientific exploration.
A pentamethine cyanine dye has been synthesised that is fluorescent when protonated, becoming non-fluorescent upon proton abstraction. The probe has a pKa of 7.5, with observable changes in the fluorescent emission properties across a pH range of 6.0–8.0, therefore providing a useful probe for studying pH in biological media.
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Briggs et al. (2000) studied this question.
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