Key result
IL-17 signaling drives aortic valve calcification through a JUN-dependent osteogenic pathway.
Why the study?
Uncertainties persist regarding how defined pro-inflammatory pathways are transcriptionally coupled to the valvular interstitial cell osteogenic program at the cell-intrinsic level in calcific aortic valve disease.
Does IL-17-associated inflammatory signaling promote calcification in aortic valve interstitial cells through a JUN-dependent mechanism?
Does IL-17-associated inflammatory signaling promote calcification in aortic valve interstitial cells through a JUN-dependent mechanism?
IL-17-associated inflammatory signaling promotes osteogenic differentiation and calcification in aortic valve interstitial cells partially through a JUN-dependent mechanism, highlighting a potential therapeutic target for calcific aortic valve disease.
IL-17/JUN signaling may promote aortic valve calcification; leaves open therapeutic targeting pending human validation.
Calcific aortic valve disease (CAVD) involves fibro-calcific remodeling, in which osteogenic differentiation of valvular interstitial cells (VICs) is a key process. Although inflammation is implicated, uncertainties persist regarding how defined pro-inflammatory pathways are transcriptionally coupled to the VIC osteogenic program at the cell-intrinsic level. We implemented an interleukin-17 (IL-17) pathway-guided transcriptomic strategy in a pure human VIC osteogenic differentiation model. RNA sequencing identified osteogenesis-associated differentially expressed genes and IL-17-related inflammatory signatures. Functional enrichment and multilayer network analyses were applied to prioritize candidate regulatory nodes, followed by in vitro validation. An IL-17-associated transcriptional program was activated during VIC osteogenic differentiation and was enriched for stress-activated and MAPK-related inflammatory pathways. Network-based analyses consistently identified the AP-1 transcription factor JUN as a central regulatory node within this IL-17-linked signature. Experimentally, JUN expression increased during VIC osteogenic differentiation, and JUN silencing attenuated calcification. IL-17 A rapidly increased the p-c-JUN/JUN ratio, and prolonged treatment upregulated JUN and IL-17RA in a concentration-dependent manner. Exogenous IL-17 A stimulation enhanced calcification-related phenotypes in VICs, which were partially reversed by JUN knockdown. In a chronic kidney disease-induced mouse CAVD model, JNK inhibition with SP600125 reduced valve calcification and leaflet thickness. IL-17-associated inflammatory signaling is engaged during VIC osteogenic differentiation and may promote calcification, partially through a JUN-dependent mechanism. These findings provide insight into how inflammatory programs interface with osteogenic transcription and offer a framework for dissecting inflammation-driven calcification in CAVD.
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Dai et al. (2026) studied Calcific aortic valve disease. IL-17A stimulation and JUN inhibition vs. Control/Vehicle was evaluated on Osteogenic differentiation and valve calcification. IL-17-associated inflammatory signaling promotes osteogenic differentiation and calcification in aortic valve interstitial cells, partially through a JUN-dependent mechanism.
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