Key result
Experimental myocardial infarction in mice led to a three-fold increase in the frequency of immature HSA+ mononucleated cardiomyocytes (4.7%) compared to sham-operated controls (1.7%).
Why the study?
Assessment of cardiac regenerative capacity has been compromised by a lack of surface signatures to characterize cardiomyocytes.
Do HSA/CD24+ immature cardiomyocytes persist in the adult heart and respond to ischemic injury?
Do HSA/CD24+ immature cardiomyocytes persist in the adult heart and respond to ischemic injury?
The identification of a persistent population of immature HSA/CD24+ cardiomyocytes that expand after ischemic injury provides new cellular targets for cardiac regeneration therapies.
Answers are grounded in this paper and cite their sources.
May identify regenerative targets in adult myocardium; hypothesis-generating and requires human validation before clinical translation.
The assessment of the regenerative capacity of the heart has been compromised by the lack of surface signatures to characterize cardiomyocytes. Here, combined multiparametric surface marker analysis with single cell transcriptional profiling and in vivo transplantation, identify the main fetal cardiac populations and their progenitors. We found that cardiomyocytes at different stages of differentiation co-exist during development. We identified a population of immature HSA/CD24 + cardiomyocytes that persists throughout life and that, unlike other cardiomyocyte subsets, actively proliferates up to one week of age and engraft cardiac tissue upon transplantation. In adult heart HSA/CD24 + cardiomyocytes appear as mononucleated cells that cycle and increase in frequency after infarction. Our work identified cell surface signatures that allow the prospective isolation of cardiomyocytes at any developmental stage and the detection of adult cardiomyocytes poised for activation in response to ischemic stimuli. This work opens new perspectives in the understanding and treatment of heart pathologies.
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Valente et al. (2019) studied Myocardial Infarction (animal model). Myocardial infarction (experimental) vs. Sham-operated was evaluated on Frequency of HSA+ mononucleated cardiomyocytes. Experimental myocardial infarction in mice led to a three-fold increase in the frequency of immature HSA+ mononucleated cardiomyocytes (4.7%) compared to sham-operated controls (1.7%).
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