Key result
The review outlines the 50-year evolution of electron microscopy from 2D imaging to 3D nano-resolution reconstructions, with a special focus on muscle ultrastructure.
This review highlights the 50-year evolution of electron microscopy techniques enabling the transition from 2D imaging to 3D nano-resolution reconstructions, particularly in muscle ultrastructure.
Technical advances enable 3D EM muscle imaging; extends research tools but leaves open clinical translation.
This is a brief and necessarily very sketchy presentation of the evolution in electron microscopy (EM) imaging that was driven by the necessity of extracting 3-D views from the essentially 2-D images produced by the electron beam. The lens design of standard transmission electron microscope has not been greatly altered since its inception. However, technical advances in specimen preparation, image collection and analysis gradually induced an astounding progression over a period of about 50 years. From the early images that redefined tissues, cell and cell organelles at the sub-micron level, to the current nano-resolution reconstructions of organelles and proteins the step is very large. The review is written by an investigator who has followed the field for many years, but often from the sidelines, and with great wonder. Her interest in muscle ultrastructure colors the writing. More specific detailed reviews are presented in this issue.
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Clara Franzini‐Armstrong (2015) conducted a review in Muscle ultrastructure. Electron microscopy was evaluated. The review outlines the 50-year evolution of electron microscopy from 2D imaging to 3D nano-resolution reconstructions, with a special focus on muscle ultrastructure.
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