Key result
Both tumor and normal lactotropes secreted RAS components, with a generally higher percentage of secretory cells in tumor lactotropes and similar subcellular distribution in both cell types.
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Distinct RAS secretion in tumoral vs normal lactotropes leaves open their role in prolactinoma behavior; human validation required before clinical relevance.
Immunodetection of renin-angiotensin system (RAS) components indicates that there is a local RAS in anterior pituitary cells, particularly in lactotropes. We have attempted to determine if RAS molecules are secreted by lactotropes and the secretory pathways and intracellular sites of maturation. We investigated the secretory activity of individual lactotropes, using the reverse hemolytic plaque assay (RHPA), with GH3B6 tumor cells and normal male rat pituitary cells. We also determined the subcellular distributions of RAS components in these cells. Both tumor and normal cells secreted angiotensinogen, prorenin, renin, angiotensin I, angiotensin-converting enzyme, and angiotensin II, although at different levels. The percentage of secretory cells was generally higher in tumor lactotropes than in normal cells. The subcellular distribution of RAS components obtained by immunoperoxidase was very similar in both cell types, although the intensities of immunoreactivity differed. Cleaved and uncleaved components were found in rough endoplasmic reticulum (RER), Golgi saccules, and secretory granules, all compartments of the secretory pathway. The cleaved components in the RER suggest the existence of early maturation, whereas the presence of uncleaved products in the secretory granules of normal lactotropes might indicate late maturation sites.
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Vila‐Porcile et al. (2000) studied this question. GH3B6 tumor cells vs. Normal male rat pituitary cells was evaluated on Secretion and subcellular distribution of RAS components. Both tumor and normal lactotropes secreted RAS components, with a generally higher percentage of secretory cells in tumor lactotropes and similar subcellular distribution in both cell types.
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