Key result
FHL2 protein expression was significantly reduced across different forms of human heart failure when profiled using tissue microarrays.
Tissue microarrays using cryopreserved human cardiac samples demonstrate a significant reduction in FHL2 protein expression in human heart failure.
New TMA platform enables high-throughput proteomics on preserved HF/donor cardiac tissue with paired molecular assays; leaves open validation for routine biobanking use.
Tissue MicroArrays (TMAs) are a versatile tool for high-throughput protein screening, allowing qualitative analysis of a large number of samples on a single slide. We have developed a customizable TMA system that uniquely utilizes cryopreserved human cardiac samples from both heart failure and donor patients to produce formalin-fixed paraffin-embedded sections. Confirmatory upstream or downstream molecular studies can then be performed on the same (biobanked) cryopreserved tissue. In a pilot study, we applied our TMAs to screen for the expression of four-and-a-half LIM-domain 2 (FHL2), a member of the four-and-a-half LIM family. This protein has been implicated in the pathogenesis of heart failure in a variety of animal models. While FHL2 is abundant in the heart, not much is known about its expression in human heart failure. For this purpose, we generated an affinity-purified rabbit polyclonal anti-human FHL2 antibody. Our TMAs allowed high-throughput profiling of FHL2 protein using qualitative and semiquantitative immunohistochemistry that proved complementary to Western blot analysis. We demonstrated a significant relative reduction in FHL2 protein expression across different forms of human heart failure.
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Lal et al. (2016) studied Heart failure. Heart failure vs. Donor patients was evaluated on FHL2 protein expression. FHL2 protein expression was significantly reduced across different forms of human heart failure when profiled using tissue microarrays.
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