Sirt1 overexpression attenuates Western‐style diet‐induced aortic stiffening in mice

Figures

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  FIGURE 1. (a) Sirt1 protein expression measured by western blotting, in thoracic aorta excised from WT and Sirt1tg mice (b) Aortic pulse wave velocity (PWV), (c) heart rate, (d) systolic blood pressure (SBP), (e) diastolic blood pressure (DBP), (f) mean arterial pressure (MAP), and the (g) passive pressure diameter relation in excised carotid arteries of WT and Sirttg littermates fed normal chow (NC) or 12 weeks of Western diet (WD). Individual data points are separated by sex are indicated in blue (male) and pink (female). *Denotes a significant difference within genotype, p < 0.05. Values are mean ± SEM

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  FIGURE 2. (a) Collagen, (b) elastin, (c) MMP2, and (d) MMP3 mRNA expression in thoracic aorta excised from WT and Sirttg mice fed either normal chow (NC) or Western diet (WD) for 12 weeks. Individual data points separated by sex are indicated in blue (male) and pink (female). *Denotes a significant difference, p < 0.05. Values are mean ± SEM

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  FIGURE 3. (a) Representative images of aortic sections stained with Movat's pentachrome (MP). (b) Collagen content and (c) proteoglycan content, assessed in MP stained sections of thoracic aorta excised from WT and Sirttg mice fed either normal chow (NC) or Western diet (WD) for 12 weeks. (d) Representative images of aortic sections stained with Verhoeff's Van Gieson (VVG). (e) Elastin breaks (arrowheads) and (f) ratio of elastin breaks in aortic medial area to aortic area in VVG stained aortic sections excised from WT and Sirttg mice fed either normal chow (NC) or Western diet (WD) for 12 weeks. Individual data points are separated by sex are indicated in blue (male) and pink (female)

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  FIGURE 4. (a) Representative images of aortic sections stained with hematoxylin and eosin (H&E). (b) Aortic diameter, (c) medial area, (d) medial thickness, (e) ratio of medial thickness to aortic diameter, and (f) ratio of nuclei count to medial area in H&E stained thoracic aorta sections excised from WT and Sirttg mice fed either normal chow (NC) or Western diet (WD) for 12 weeks. Individual data points are separated by sex are indicated in blue (male) and pink (female)

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  FIGURE 5. (a) Blood glucose during a glucose tolerance test (GTT) (2 g/kg, ip), and (b) area under curve (AUC) during the GTT (c) insulin tolerance test (ITT) (1 U/kg, ip). Individual data points are separated by sex are indicated in blue (male) and pink (female). *Denotes a significant difference, p < 0.05. Values are mean ± SEM