Lifelong SIRT-1 overexpression attenuates large artery stiffening with advancing age

Figures

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  Figure 1. Aortic SIRT-1 mRNA gene expression (A) in wild-type (WT) and SIRT-1 transgenic overexpressing (SIRTTG) mice (N=4-8/group) normalized to Young WT. Scatter plot (B) of aortic pulse wave velocity (PWV) with advancing age in WT and SIRTTG mice. Separate regression lines are given for WT and SIRTTG mice (dotted lines define 95% confidence intervals). Aortic PWV measured at 3 mo increments in WT (C) and SIRTTG (D) mice (N=7-24/group). *P<0.05 vs. WT in same age group. †P<0.05 vs. Young. a, b, c, d, e, f P<0.05 vs. 3, 6, 9, 12, 15, 18, 21 mo, respectively. Data are mean±SEM.

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  Figure 2. Thoracic aorta lumen diameter (A), medial cross-sectional area (B [CSA]), and media-to-lumen ratio (C) in young, middle-age, and old wild-type (WT) and SIRT-1 transgenic overexpressing (SIRTTG) mice. *P<0.05 vs. WT in same age group. †P<0.05 vs. Young. ‡P<0.05 vs. Middle-Age. Data are individual values and means.

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  Figure 3. Total thoracic aortic elastin (A), collagen (B), and advanced glycation end products (C [AGEs]) content in young, middle-age, and old wild-type (WT) and SIRT-1 transgenic overexpressing (SIRTTG) mice. Figures are accompanied by representative images of elastin, collagen, and AGEs staining. Black scale bars are equal to 200 μm. *P<0.05 vs. WT. †P<0.05 vs. Young. ‡P<0.05 vs. Middle-Age. Data are individual values and means normalized to Young WT.

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  Figure 4. Calcified area relative to tunica media area in young, middle-age, and old wild-type (WT) and SIRT-1 transgenic overexpressing (SIRTTG) mice. Figure is accompanied by representative images. Black scale bars are equal to 100 μm. *P<0.05 vs. WT. †P<0.05 vs. Young. Data are individual values and means.

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  Figure 5. Aortic SOD1, SOD2, and SOD3 mRNA gene expression in young and old wild-type (WT) and SIRT-1 transgenic overexpressing (SIRTTG) mice (N=5-7/group). *P<0.05 vs. Young WT. †P<0.05 vs. Young SIRTTG. ‡P<0.05 vs. Old WT. Data are mean±SEM normalized to Young WT.