Key result
Acute ischemic stroke patients exhibited significantly smaller platelet dense granules (CD63 average size 0.69 vs 1.45 μm2, p=0.038) and α granules compared to healthy controls.
Why the study?
Observing and evaluating platelet ultrastructure and antiplatelet drug efficacy in AIS patients is difficult due to microscopic limitations and platelet sensitivity.
Does super-resolution microscopy reveal differences in platelet ultrastructure and function in patients with acute ischemic stroke compared to healthy controls?
Cross-Sectional (n=19)
No
Does super-resolution microscopy reveal differences in platelet ultrastructure and function in patients with acute ischemic stroke compared to healthy controls?
Absolute Event Rate: 0.69% vs 1.45%
p-value: p=0.038
Super-resolution microscopy can detect altered platelet ultrastructure and function in acute ischemic stroke patients, offering a potential new method for assessing antiplatelet drugs.
SIM enables rapid platelet ultrastructure assessment in AIS; leaves open whether findings guide antiplatelet therapy.
Platelets are important in acute ischemic stroke (AIS) thrombosis. The observation and evaluation of platelet ultrastructure and efficacy of antiplatelet drug in AIS patients is difficult due to microscopic limitations and sensitivity of platelet. The new super-resolution microscope (SIM) can accurately, quickly analyze the platelet ultrastructure and antiplatelet drug in AIS patients. We applied SIM to observe the morphology and ultrastructure of platelets with AIS patients in different state. SIM images were analyzed to specify the dense granules and α granules change of platelets in AIS patients. Testing platelet factor 4 (PF4) to reflect platelet releasing function. We observed that platelet activation in AIS patients was greater after stimulation, with α granule showing a pattern of parenchymatous masses. SIM images analyzing showed diameter of platelets, average size of granules, area% of granules per field and mean area of granules per platelet in AIS patient were lower than healthy people. Platelet releasing function was suppressed at rest stage and more efficacy release after stimulation. 2MeSamp inhibited parenchymatous masses of α granules and reduced PF4 release of platelets after stimulation. According to the results, the structure and function of platelets in AIS patients are indeed altered. Additionally, SIM could be used as a new method to indicates the onset of AIS and assess antiplatelet drugs.
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Yang et al. (2022) conducted a cross-sectional in Acute Ischemic Stroke (n=19). Acute ischemic stroke vs. Healthy controls was evaluated on CD63 average size (dense granules) (p=0.038). Acute ischemic stroke patients exhibited significantly smaller platelet dense granules (CD63 average size 0.69 vs 1.45 μm2, p=0.038) and α granules compared to healthy controls.
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