What question did this study set out to answer?

This research aims to investigate the expression and functionality of the dihydrofolate reductase-like 1 (DHFRL1) gene in humans.

The former annotated human pseudogene dihydrofolate reductase-like 1 ( DHFRL1 ) is expressed and functional

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Randomized trial shows expression and function of DHFR-like gene in human cells, suggesting new metabolic roles.

This research aims to investigate the expression and functionality of the dihydrofolate reductase-like 1 (DHFRL1) gene in humans.
Identified and characterized the DHFRL1 gene on chromosome 3.
Used recombinant DHFRL1 in bacterial and mammalian cells to analyze its functional capacity.
Conducted kinetic assays to compare DHFRL1 and DHFR activity and substrate affinities.
Recombinant DHFRL1 complemented a DHFR-negative phenotype, indicating its functional capacity (specific activity lower than DHFR).
K(m) values for NADPH were similar, but DHFRL1 showed a higher K(m) for dihydrofolate compared to DHFR.
Confocal microscopy revealed DHFRL1 localization in mitochondria, suggesting a unique role in mitochondrial folate metabolism.

McEntee et al. (2011) studied this question.

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