Answers are grounded in this paper and cite their sources.
Lipofuscin pigment determination in tissue extracts was quantitated by the use of its property of fluorescence. Chloroform:methanol tissue extracts were purified on Sephadex LH-20 columns before quantitative fluorescence measurements of the lipofuscin pigments. Interfering compounds separated by chromatography were retinol and a lower mol wt fluorescent compound. Irradiation of tissue extracts with ultraviolet light was not sufficient to eliminate the interference caused by retional and the lower mol wt compound. Purified lipofuscin pigments from blood, lung, liver, spleen, brain, heart, and kidney tissues demonstrated distinct fluorescent emission maximum at 435 nm and excitation maxima between 345 and 350 nm.
No takes yet. Share an insight, caveat, or question.
Csallany et al. (1976) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: