Key result
Two CA16 virion fractions (real and pseudo-virion) showed similar pathogenicity in a neonatal murine model and induced an effective primary immune response in adult mice.
Both real and pseudo-virion fractions of CA16 demonstrate similar pathogenicity and immunogenicity in murine models, despite structural differences in VP0 cleavage.
Animal data on CA16 virion fractions leave open differential human pathogenicity and immunogenicity; clinical studies needed.
OBJECTIVES: To investigate the biological characteristics of the two types of virion fractions of Coxsackievirus A 16 (CA16), which include the real virion fraction and pseudo-virion fraction in their structure, pathogenicity and immunogenicity. METHODS: We obtained the two CA16 virion fractions by density gradient centrifugation. The morphology of virion fractions was analyzed by electron microscopy, while the antigenic characteristics and immunogenicity of two virion fractions were determined by ELISA, SDS-PAGE, Western blot, qRT-PCR, and the mouse model of immune response. RESULTS: The two virion fractions contained the major viral antigen components in their structures, showed similar pathogenicity in a neonatal murine model and were capable of inducing an effective primary immune response in adult mice, regardless of the essential distinction between the two virion fractions, which was the cleavage of VP0 to VP2 and VP4. CONCLUSIONS: The two CA16 virion fractions showed antigenicity and immunogenicity with inducing a specific immune response in animals.
No takes yet. Share an insight, caveat, or question.
Pu et al. (2015) studied Coxsackievirus A 16 (CA16) infection. Two virion fractions of Coxsackievirus A 16 (real virion fraction and pseudo-virion fraction) was evaluated on Structure, pathogenicity, and immunogenicity. Two CA16 virion fractions (real and pseudo-virion) showed similar pathogenicity in a neonatal murine model and induced an effective primary immune response in adult mice.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: