NIR‐II‐Driven Multiphoton Excited Afterglow Luminescence in Metal‐Organic Frameworks for Tumor Diagnostics

Near‐infrared (NIR) light‐driven afterglow luminescence probes hold significant value for precise tumor diagnosis. However, current studies are predominantly focused on the first NIR window (NIR‐I), while the second NIR window (NIR‐II) remains largely unexplored. Herein, we report the development of the first NIR‐II‐driven afterglow luminescence nanoprobe based on a multiphoton platform. This nanoprobe ( UTDMH ) consists of a multiphoton‐harvesting metal‐organic framework ( UiO‐66‐Th ) loaded with an afterglow luminescence substrate ( DO ), capable of generating singlet oxygen under 1200 nm irradiation, thereby triggering afterglow luminescence. Furthermore, it integrates lysosome‐targeting and pH‐responsive capabilities, which allows precise “on‐to‐off” regulation of afterglow luminescence signals within the acidic lysosomal microenvironment of tumor cells, facilitating precise tumor diagnosis. This work thus establishes a general strategy for constructing NIR‐II‐activatable afterglow luminescence nanoplatforms.