Сhemical and biological properties of sodium fullerenolates from an undivided mixture of fullerenes

Obtaining pharmaceuticals from an undivided fullerite mixture streamlines production processes and reduces costs. However, it also increases variability and the number of compounds with nearly identical chemical properties, although not necessarily similar biological effects. Therefore, it is essential to produce a mixture of sodium fullerenolates with consistent biological properties, considering their promising potential in medical applications for treating serious diseases. The primary objective of this study was to investigate the chemical and biological characteristics of new fullerene derivatives. Specifically, a chemical analysis of these novel carbon samples was performed. Additionally, secondary objectives included evaluating their potential biological effects on the contractile activity of the mouse intestine and assessing their biotoxicity. A set of sodium fullerenolates samples with stable physicochemical properties was examined for their biological effects and toxicity. Results indicated that fullerenolates inhibit depolarization-induced contractions of the mouse small intestine, while they do not influence carbachol-induced or spontaneous contractions. Characterization of sodium fullerenolates derived from a mixture of fullerenes using physicochemical methods demonstrated complete non-toxicity and revealed several biological properties of interest for biomedical research.