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Highly sensitive and selective microRNA (miRNA) assay is of great significance for disease diagnosis and therapy. Herein, a magnetic-assisted electrochemistry (EC)–photoelectrochemistry (PEC) dual-mode biosensing platform was developed for miRNA-210 detection based on dual-signaling EC and photocurrent-polarity-switching PEC strategies. Porous magnetic Fe3O4 octahedra with a large surface area were synthesized by calcining Fe-based metal–organic frameworks. Subsequently, the magnetic photoelectric materials (Fe3O4@CdS) were developed by the successive ionic layer adsorption and reaction method in Cd2+ and S2– solutions. Then, the self-assembled DNA nanoprisms contained three thiols/hanging arms that could capture miRNA-210 efficiently and were anchored to the Fe3O4@CdS octahedra via the Cd–S bond. When miRNA-210 was present, the double-stranded DNA concatemers the self-assembled duplex helixes based on a pair of methylene blue (MB)-labeled single-stranded DNAs (AP1 and AP2) through the hybridization chain reaction and then intercalated with adriamycin (Dox) into their grooves were connected with the Fe3O4@CdS-DNA nanoprisms. MB and Dox not only acted as the electrochemical probes but also synergistically switched the photocurrent polarity of the Fe3O4@CdS octahedra. Thus, miRNA-210 was assayed sensitively and selectively via the proposed EC–PEC dual-mode biosensing platform. Additionally, the abovementioned recognition steps occurred in a homogeneous system, and the effects of the impurities and interferences on the miRNA-210 assay could be easily avoided by magnetic separation due to the good magnetic properties of Fe3O4 octahedra. The proposed EC–PEC dual-mode biosensing platform showed a wide range of potential applications in bioanalysis and early diagnosis of disease.
Zhang et al. (Wed,) studied this question.