Differential expression profiles and functional analysis of long non-coding RNAs in calcific aortic valve disease

Figures

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  Fig. 1. Characterization of lncRNAs compared with protein-coding genes Comparison of (A) transcript length; (B) numbers of exons; (C) exon length; (D) expression level

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  Fig. 2. DEGs and DELs are identified in differential expression analysis PCA is based on (A) mRNA and (B) lncRNA. Volcano plots of (C) DEGs and (D) DELs. GO analysis of (E) upregulated DEGs and (F) downregulated DEGs

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  Fig. 3. Identifying the key module associated with AS (A) Total genes were clustered into 25 modules, and each module is marked with one color. (B) Heatmap of distances showing obvious discrimination among modules. (C) Module gene significance in relation to AS. (D) GO analysis of midnightblue module

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  Fig. 4. Cis- and trans-regulatory functions of lncRNAs in the midnightblue module (A) Heat plot of GO enrichment of cis-mRNA in midnightblue module. The black cells in the heat map represent the GO terms that were enriched by cis-mRNAs, while the white cells represent the GO terms that were not enriched by the cis-mRNAs; (B) number of trans-regulated protein-coding genes by lncRNAs in midnightblue module; (C) enrichment score (ES) of GO enrichment of mRNAs trans-regulated by lncRNAs; (D) association of lncRNAs, their coexpressed mRNAs, and GO enrichment terms. Width of edges between GO terms and lncRNAs related to the number of potential mRNAs trans-regulated by lncRNAs enriched in GO terms

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  Fig. 5. Potential functions of lncRNAs through ceRNA mechanisms in midnightblue module GO enrichment analysis for mRNAs regulated by (A) MIR4435-2HG; (B) FAM225A; and (C) BHLHE40-AS1 through a ceRNA mechanism

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  Fig. 6. ROC curves analysis of hub cis- or trans-regulatory genes AUC based on each of the top 3 hub genes in (A) GSE148219 (B) GSE199718