Directed evolution of the heme domain of cytochrome P450 BM-3 has resulted in a versatile, highly active peroxide-driven hydroxylation catalyst (see picture) that requires neither NADPH nor reductase and functions in a cell-free reaction system. This simplified, “biomimetic” catalyst is amenable to further optimization, for example, to improve stability or alter its substrate range.
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Cirino et al. (2003) studied this question.
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