Key result
Incrementing resting tension up to 1.61 gf significantly augmented 40 mmol/L KCl stimulated tension in human pulmonary artery rings, establishing 1.61 gf as the optimal resting tension.
The optimal resting tension for in vitro experiments using human pulmonary artery rings is 1.61 gf, which maximizes contractile responses to KCl.
Optimizes human PA ring protocols for maximal KCl response; leaves open translation to clinical pulmonary vascular studies.
AIM: To determine the optimum resting tension (ORT) for in vitro human pulmonary artery (PA) ring preparations. METHODS: ) to measure changes in isometric tension. The resting tension was set at 1-gram force (gf) with vessels being left static to equilibrate for duration of one hour. Baseline contractile reactions to 40 mmol/L KCl were obtained from a resting tension of 1 gf. Contractile reactions to 40 mmol/L KCl were then obtained from stepwise increases in resting tension (1.2, 1.4, 1.6, 1.8 and 2.0 gf). RESULTS: Twenty PA rings of internal diameter between 2-4 mm were prepared from 4 patients. In human PA rings incrementing the tension during rest stance by 0.6 gf, up to 1.6 gf significantly augmented the 40 mmol/L KCl stimulated tension. Further enhancement of active tension by 0.4 gf, up to 2.0 gf mitigate the 40 mmol/L KCl stimulated reaction. Both Myograph and the organ bath demonstrated identical conclusions, supporting that the radial optimal resting tension for human PA ring was 1.61 g. CONCLUSION: The radial optimal resting tension in our experiment is 1.61 gf (15.78 mN) for human PA rings.
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Hussain et al. (2016) studied this question. Stepwise increases in resting tension vs. Baseline resting tension of 1 gf was evaluated on Contractile reactions to 40 mmol/L KCl. Incrementing resting tension up to 1.61 gf significantly augmented 40 mmol/L KCl stimulated tension in human pulmonary artery rings, establishing 1.61 gf as the optimal resting tension.
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