Key result
Mice overexpressing renin and angiotensinogen showed a larger infarct volume (22.2% vs 14.1%, P<0.01) and higher neurological deficit scores following middle cerebral artery occlusion.
Overexpression of renin and angiotensinogen exacerbates ischemic brain injury in mice, an effect abolished by losartan, indicating that ANG II/AT1 signaling is harmful during cerebral ischemia.
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Cautions against RAS overactivation in experimental ischemia; leaves open human translation and AT1 blockade trials.
To investigate the role of brain angiotensin II (ANG II) in the pathogenesis of injury following ischemic stroke, mice overexpressing renin and angiotensinogen (R+A+) and their wild-type control animals (R-A-) were used for experimental ischemia studies. Focal brain ischemia was induced by middle cerebral artery occlusion (MCAO). The severity of ischemic injury was determined by measuring neurological deficits and histological damage at 24 and 48 h after MCAO, respectively. To exclude the influence of blood pressure and local collateral blood flow, brain slices were used for oxygen and glucose deprivation (OGD) studies. The severity of OGD-induced damage was determined by measuring indicators of tissue swelling and cell death, the intensity of the intrinsic optical signal (IOS), and the number of propidium iodide (PI) staining cells, respectively. Results showed 1) R+A+ mice showed higher neurological deficit score (3.8 +/- 0.5 and 2.5 +/- 0.3 for R+A+ and R-A-, respectively, P < 0.01) and larger infarct volume (22.2 +/- 1.6% and 14.1 +/- 1.2% for R+A+ and R-A-, respectively, P < 0.01); 2) The R+A+ brain slices showed more severe tissue swelling and cell death in the cortex (IOS: 140 +/- 6% and 114 +/- 10%; PI: 139 +/- 20 cells/field and 39 +/- 9 cells/field for R+A+ and R-A-, respectively, P < 0.01); 3) treatment with losartan (20 micromol/l) abolished OGD-induced exaggeration of cell injury seen in R+A+ mice. The data indicate that activation of ANG II/AT(1) signaling is harmful to brain exposed to ischemia.
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Chen et al. (2009) studied Ischemic stroke. Overexpression of renin and angiotensinogen (R+A+) vs. Wild-type control animals (R-A-) was evaluated on Infarct volume (p=<0.01). Mice overexpressing renin and angiotensinogen showed a larger infarct volume (22.2% vs 14.1%, P<0.01) and higher neurological deficit scores following middle cerebral artery occlusion.
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