The fluorescence decay time (tau) was 2 to 5 nanoseconds for proteins and 4 to 5 nanoseconds for flavin, pyridine nucleotide, and vitamin B(6)coenzymes; tau varied widely in 48 compounds measured in water. Altholugh reported values of tau for a few of the soluttions studied were in excellenlt agreement. previously "calculated" lifetimes, in severall instances, are apparently erroneous. Nonexponential decay was detectable with our "nanosecond-flash" apparatus, a modification of the first commercially aavilable unit for determination of tau.
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Chen et al. (1967) studied this question.
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