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June 20, 2011Molecular Ecology ResourcesOpen Access

DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer

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Authors

GRG.P. RobideauUniversity of California, RiversideACArthur W. A. M. de CockWesterdijk Fungal Biodiversity InstituteMCMichael D. CoffeyNottingham Trent University

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Implication

Randomized trial assesses DNA barcoding efficacy for oomycete identification, indicating reliability of COI and ITS.

Key Points

  • This study aims to evaluate the efficacy of cytochrome c oxidase subunit I (COI) for DNA barcoding of oomycete species.
  • Sequenced cytochrome c oxidase subunit I (COI) from 1205 isolates spanning 23 genera of oomycetes.
  • Compared COI sequences to internal transcribed spacer (ITS) sequences from the same isolates.
  • Assessed species resolution capabilities of COI, ITS, and large ribosomal subunit sequencing.
  • COI identification proved to be as practical and complementary to ITS, enhancing species-level discrimination.
  • In some instances, COI was more effective than ITS for distinguishing between species.
  • The large ribosomal subunit sequencing showed poor resolution at the species level compared to COI and ITS.

Cite This Study

Robideau et al. (2011) studied this question.

synapsesocial.com/papers/6a63cdf213c4f809e6385352https://doi.org/10.1111/j.1755-0998.2011.03041.x
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