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February 1, 1987Genetics

Protease B of Saccharomyces cerevisiae: Isolation and Regulation of the PRB1 Structural Gene

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Authors

CMCharles M. MoehleMAMartha W AynardiMKMichael R Kolodny

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Overview

Randomized trial investigates PRB1 gene function in yeast, suggesting complex transcriptional regulation and proteolysis.

Key Points

  • The aim is to isolate and characterize the PRB1 gene responsible for protease B in yeast.
  • Isolated the PRB1 gene from a genomic library via complementation of the prb1-1122 mutation.
  • Conducted deletion analysis to localize functional activity to a specific DNA fragment.
  • Performed S1 endonuclease mapping to confirm colinearity between mRNA and gene.
  • Identified a 2.3-kb mRNA with potential to encode a 69,000 molecular weight protein.
  • Observed that PRB1 mRNA levels increase when glucose is depleted during diauxic growth phase.
  • Constructed a deletion that removes 83% of the coding region, resulting in no significant phenotype except protease B deficiency.

Cite This Study

Moehle et al. (1987) studied this question.

synapsesocial.com/papers/6a63dea194d8bae735b36fc4https://doi.org/10.1093/genetics/115.2.255
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  1. 1The <i>PEP4</i> Gene Encodes an Aspartyl Protease Implicated in the Posttranslational Regulation of <i>Saccharomyces cerevisiae</i> Vacuolar Hydrolases1986 · 282 citations
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