Biosensors of single-gene knockout mutants and physical methods using mesh and quartz glass are employed to discriminate plasma agents and assess their lethal effects generated in a Direct-Current atmospheric-pressure oxygen plasma jet. Radicals generated in plasma are determined by optical emission spectroscopy, along with the O 3 density measurement by UV absorption spectroscopy. Besides, thermal effect is investigated by an infrared camera. The biosensors include three kinds of Escherichia coli (E. coli) K-12 substrains with their mutants, totalling 8 kinds of bacteria. Results show that oxidative stress plays a main role in the inactivation process. Rather than superoxide O 2 - , neutral reactive oxygen species such as O 3 and O 2 ( a 1 Δ g ) are identified as dominant sources for oxidative stress. In addition, DNA damage caused by oxidation is found to be an important destruction mechanism.
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Li et al. (2013) studied this question.
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