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Plasma-activated water (PAW) is an eco-friendly technology with potential to improve seed germination and nutritional quality in microgreens. This study investigated the effects of PAW on three cultivars of kangkong (Ipomoea aquatica Forssk.). PAW activated for 10 min (PAW10) significantly enhanced seed germination and vigor, with effects comparable to those of a 15-min treatment. PAW10 treatment not only improved the accumulation of bioactive compounds—including total phenolics, flavonoids, ascorbic acid, chlorophylls, and carotenoids—but also enhanced antioxidant activity. These improvements were accompanied by elevated hydrogen peroxide (H2O2) levels and increased enzymatic activities, specifically catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX). Principal component analysis revealed cultivar-specific responses to PAW10. The Senafore 20 (SF) cultivar showed the most pronounced increases in antioxidant and antiglycation activities, as well as key bioactive compounds. The Phai-ngern (PN) cultivar exhibited elevated SOD activity and fiber content, while the Senee 20 (SN) cultivar showed minimal changes. These findings suggest that PAW10 effectively promotes germination and antioxidant-related biochemical responses in kangkong microgreens, with varying responses depending on cultivar. This study highlights PAW treatment as a promising approach to improve microgreen production and antioxidant capacity, supporting sustainable agriculture.
Ongrak et al. (Fri,) studied this question.