Key result
CRISPR/Cas systems demonstrate simplicity, rapidity, high efficiency, and specificity for nucleic acid detection, highlighting their potential in point-of-care testing.
Why the study?
CRISPR technology has demonstrated potential for rapid, simple, and specific nucleic acid detection to meet the needs of point-of-care testing and bedside monitoring.
This review summarizes the principles, applications, and challenges of CRISPR/Cas systems in rapid nucleic acid detection and point-of-care testing.
May support rapid POC nucleic acid testing in practice; leaves open prospective validation before clinical adoption.
Clustered regularly interspaced short palindromic repeats (CRISPR) technology, as a powerful tool for gene editing and gene regulation, has been widely developed and applied in life science, biotechnological industry, gene therapy and other fields. In recent years, based on specific and nonspecific nuclease activity of CRISPR-associated protein (Cas protein), CRISPR technology has shown its characteristics of simplicity, rapidity, high efficiency and specificity for nucleic acid detection and its great application potential in point-of-care testing (POCT), which can meet the needs of rapid diagnosis for early clinical treatment and bedside monitoring. According to the different activities of Cas protein (cis-cleavage activity and trans-cleavage activity), we classify CRISPR/Cas systems used for nucleic acid detection into two classes, CRISPR/Cas9 system and CRISPR/Cas12, Cas13, Cas14 system, and explain their working principles respectively. Besides, the article reviews the combined applications of CRISPR/Cas systems and multiple signal transducers in detail. The current shortcomings and challenges of all these CRISPR-based technologies are summarized elaborately and their future prospects are outlined.
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ZHAO et al. (2023) conducted a review in Nucleic acid detection. CRISPR/Cas systems was evaluated. CRISPR/Cas systems demonstrate simplicity, rapidity, high efficiency, and specificity for nucleic acid detection, highlighting their potential in point-of-care testing.
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