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April 1, 1987British Journal of PharmacologyOpen Access

Endothelium‐derived relaxing factor inhibitsin vitroplatelet aggregation

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Key result

Endothelium-derived relaxing factor, bovine retractor penis muscle inhibitory factor, and sodium nitroprusside all significantly inhibited in vitro human platelet aggregation induced by collagen or U46619.

Why the study?

Does endothelium-derived relaxing factor (EDRF) inhibit in vitro platelet aggregation?

Population

Washed human platelets (in vitro model)

Design

Preclinical

Authors

BFBrenda FurlongMorehouse School of MedicineAHA.H. HendersonPrincess Alexandra HospitalMLMalcolm LewisRutgers, The State University of New Jersey

Discussion

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Implication

Highlights guanylate cyclase stimulation for platelet inhibition; leaves open in vivo thrombosis relevance.

Key Points

  • The study aims to evaluate how endothelium-derived relaxing factor (EDRF) affects platelet aggregation.
  • Investigated the effects of EDRF, bovine retractor penis muscle inhibitory factor, and sodium nitroprusside on washed human platelets.
  • Examined platelet aggregation induced by collagen and thromboxane A2 analogue U46619.
  • Assessed the influence of haemoglobin and superoxide dismutase on EDRF's effects.
  • All three agents inhibited platelet aggregation induced by collagen and U46619.
  • Haemoglobin inhibited the anti-aggregatory effect of each agent.
  • The anti-aggregatory effect of EDRF was enhanced by superoxide dismutase.

Structured PICO

Does endothelium-derived relaxing factor (EDRF) inhibit in vitro platelet aggregation?

P
Population
In vitro study using washed human platelets to assess the anti-aggregatory effects of endothelium-derived relaxing factor, bovine retractor penis muscle inhibitory factor, and sodium nitroprusside.
I
Intervention
Endothelium-derived relaxing factor (EDRF), bovine retractor penis muscle inhibitory factor, and sodium nitroprusside
O
Outcome
In vitro platelet aggregation induced by collagen or thromboxane A2 analogue U46619surrogate

Main Result

p-value: p=<0.001

EDRF and other guanylate cyclase stimulants inhibit platelet aggregation in vitro, suggesting a potential role for EDRF in haemostasis.

Limitations

  • In vitro study design
  • Short half-life of EDRF makes it difficult to assess downstream effects in intact circulation

Cite This Study

Furlong et al. (1987) studied this question. Endothelium-derived relaxing factor (EDRF), bovine retractor penis muscle inhibitory factor, and sodium nitroprusside vs. Control (absence of these agents or presence of hemoglobin) was evaluated on In vitro platelet aggregation induced by collagen or U46619 (rate and extent) (p=<0.001). Endothelium-derived relaxing factor, bovine retractor penis muscle inhibitory factor, and sodium nitroprusside all significantly inhibited in vitro human platelet aggregation induced by collagen or U46619.

synapsesocial.com/papers/6a64b03cebf08ab368f01145https://doi.org/10.1111/j.1476-5381.1987.tb11221.x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Role of the intima in cholinergic and purinergic relaxation of isolated canine femoral arteries.1981 · 380 citations
  2. 2PHARMACOLOGICAL INTERACTIONS BETWEEN PROSTACYCLIN AND THROMBOXANES1983 · 118 citations
  3. 3Autoradiographic localization of β‐adrenoceptor subtypes in guinea‐pig kidney1985 · 33 citations
  4. 4Cyclic GMP mediates neurogenic relaxation in the bovine retractor penis muscle1984 · 105 citations
  5. 5The interaction of sodium nitroprusside with human endothelial cells and platelets: nitroprusside and prostacyclin synergistically inhibit platelet function.1982 · 81 citations