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The Sandstedt, Kneen & Blish method for the assay of α-amylase uses as a measure of enzyme activity the reciprocal of the time taken for a standard decrease to occur in the starch-iodine blue colour of an incubation mixture containing a large quantity of added β-amylase. This method has been modified so that a photoelectric colorimeter is employed in conjunction with a standard graph. The precision of the method is thereby Improved and the work needed for each assay reduced. The principles involved could easily be applied to other enzyme assays.
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D. E. Briggs (1961) studied this question.
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