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In this chapter several aspects of the one‐stage assay for Factor VIII coagulant activity (VIII:C) are discussed, based both on a survey of the literature and on experimental data. Special attention is paid to the variables present in the assay system and their influence on coagulation times and Factor VIII estimates. Recommendations are given on how to carry out the assay properly and finally two examples of an assay system are presented.
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J Over (1984) studied this question.
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