ANALYSIS OF IgG HEAVY CHAIN SUBCLASSES OF ALLOANTIBODIES TO FACTOR IX BY CROSSED IMMUNOELECTROPHORESIS OF FACTOR IX USING THE INTERMEDIATE GEL TECHNIQUE

Recently, Qrstavik (198 1) reported the immunological properties of the immune complexes of factor IX and anti-factor IX antibody characterized by crossed immunoelectrophoresis (CIE) of factor IX using the intermediate gel technique. She demonstrated that all five alloantibodies to factor IX were polyclonal immunoglobulin G(IgG) antibodies. Pig 1. Crossed immunoelectrophoresis of factor IX. The intermediate gel contained 20% antiserum to human IgGl (A). 20% antiserum to human IgGz (B). 20% antiserum to human IgG3 (C) and 20% antiserum to human IgG4 (D).