Purification and N-terminal amino acid sequence of Enterocin CRL 35, a ‘pediocin-like’ bacteriocin produced by Enterococcus faecium CRL 35

Enterocin CRL 35, a bacteriocin produced by Enterococcus faecium CRL 35 that inhibits food-borne pathogens, was purified by precipitation with (NH4)2SO4, gel filtration, ion exchange and reverse phase chromatography. The partial N-terminal amino acid sequence indicated a strong homology with other 'pediocin-like bacteriocins' previously described.