Key result
Incubation with nitric oxide donors reversibly inhibits dimethylarginine dimethylaminohydrolase (DDAH) activity via S-nitrosylation of a conserved Cys-His-Glu catalytic triad.
Population
Recombinant DDAH, mammalian DDAH in cytosolic extracts, and cultured endothelial cells heterologously…
Design
Preclinical
Authors
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May modulate ADMA in NO-rich settings; leaves open in vivo relevance and any therapeutic implications.
S-nitrosylation of DDAH by nitric oxide reversibly inhibits its activity, suggesting a feedback mechanism where increased NO leads to ADMA accumulation and subsequent NOS inhibition.
Leiper et al. (2002) studied this question. Nitric oxide (NO) donors was evaluated on DDAH enzyme activity and S-nitrosylation. Incubation with nitric oxide donors reversibly inhibits dimethylarginine dimethylaminohydrolase (DDAH) activity via S-nitrosylation of a conserved Cys-His-Glu catalytic triad.
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