Changes in the Expression of MIF and Other Key Enzymes of Energy Metabolism in the Myocardia of Broiler Chickens with Ascites Syndrome

Figures

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  Figure 1. Autopsy results of AS broilers: (A) accumulation of large amounts of yellowish and clear fluid in the abdominal cavity without any fibrous clots; (B) the right ventricle shows flaccidity and poor elasticity; (C) pericardial effusion was observed in the heart.

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  Figure 2. HE of broiler myocardial tissues (40×): (A) control group; (B,C) ascitic group, showing that, in some myocardial cells, the structure of myofibrils was disordered, and a large number of blood cells were deposited in the blood vessels.

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  Figure 3. The protein expressions of MIF and p-AMPK in the control group and AS group were measured using Western blotting: (A) MIF; (B) p-AMPK and AMPK. The data are presented as the means ± SEM. The experiment was repeated three times independently. * p < 0.05 indicates a significant difference from the control group.

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  Figure 4. The protein expressions of key enzymes of the energy metabolic pathway of the myocardial tissues in ascitic broilers were measured using Western blotting: (A) the key enzymes PKM2, HK2, PFK1, and PFK2 in the glycolytic pathway of the ascitic broilers’ myocardial tissue; (B) the key enzymes OGDH, IDH2, and CS in the TCA cycle of the ascitic broilers’ myocardial tissue; (C) the key enzymes CPT-1A and p-ACC of the fatty acid oxidation pathway of the ascitic broilers’ myocardial tissue; (D) two important enzymes, PDHE1 and LDHB, of the glucose metabolism of the ascitic broilers’ myocardial tissue. The data are presented as means ± SEM. The experiment was repeated three times independently. * p < 0.05 and ** p < 0.01 indicate a significant difference from the control group.

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  Figure 5. Schematic diagram of the mechanism of the key enzymes of myocardial energy metabolism.