Randomized trial finds thrombin induces rapid degradation of phosphatidylinositol 4,5-bisphosphate in platelets, indicating its role in platelet activation.
Key Points
This study investigates the effect of thrombin on phosphatidylinositol 4,5-bisphosphate levels in platelets.
Horse platelets were prelabeled with 32P and stimulated with thrombin.
Radioactive measurements were taken to assess the degradation of phosphatidylinositol 4,5-bisphosphate.
The effect of the calcium ionophore A23187 was evaluated for comparison.
A rapid decrease of 15-20% in phosphatidylinositol 4,5-bisphosphate was observed after 10 seconds of thrombin stimulation.
No significant change in phosphatidylinositol 4-monophosphate was noted during this time.
The loss of phosphatidylinositol 4,5-bisphosphate was concentration-dependent upon thrombin stimulation.