What is the clinical evidence from this study?

Study design: Other. Population: Insulin and growth hormone signaling. Intervention: Sphingosine vs. Control (no sphingosine). Primary outcome: Hormone-stimulated lipogenesis (incorporation of tritiated glucose into lipids).

June 1, 1989Proceedings of the National Academy of SciencesOpen Access

Sphingosine, an inhibitor of protein kinase C, suppresses the insulin-like effects of growth hormone in rat adipocytes.

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Key result

Sphingosine at 50 μM decreased the lipogenic effects of human growth hormone by 65% and insulin by 89% in rat adipocytes.

Population

rat adipocytes preincubated without hGH for 4 hr

Comparison

sphingosine vs basal conditions, N-Acetylsphingosine, and…

Design

Preclinical

Authors

JSJean SmalCity Of Hope National Medical Center PMPierre De Meytsde Duve Institute

Discussion

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Implication

Sphingosine inhibits stimulated lipogenesis in rat adipocytes; leaves open PKC mediation in human metabolism.

Structured PICO

Population

In vitro study using adipocytes isolated from male Wistar rats to evaluate the effects of sphingosine on hormone-stimulated lipogenesis.

Intervention

sphingosine (50 microM, 100 microM, up to 200 microM)

Comparator

basal conditions, N-Acetylsphingosine, and sphingomyelin

Outcome

lipogenesissurrogate

Sphingosine inhibits lipogenesis and glucose uptake in rat adipocytes, providing evidence for the involvement of protein kinase C in the mechanism of action of growth hormone and insulin.

Limitations

In vitro study on rat adipocytes, which may not fully translate to in vivo human physiology
Sphingosine may have non-specific effects on cell membranes or other enzymes at high concentrations

Cite This Study

Smal et al. (1989) studied Insulin and growth hormone signaling. Sphingosine vs. Control (no sphingosine) was evaluated on Hormone-stimulated lipogenesis (incorporation of tritiated glucose into lipids). Sphingosine at 50 μM decreased the lipogenic effects of human growth hormone by 65% and insulin by 89% in rat adipocytes.

synapsesocial.com/papers/6a652fe9bc65bb50da2f068a https://doi.org/10.1073/pnas.86.12.4705

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Sphingosine, an inhibitor of protein kinase C, suppresses the insulin-like effects of growth hormone in rat adipocytes.

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Sphingosine, an inhibitor of protein kinase C, suppresses the insulin-like effects of growth hormone in rat adipocytes.

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider