Growth factors rapidly induce expression of the glucose transporter gene.
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Overview
Randomized trial shows growth factors enhance glucose transporter gene expression in fibroblast cells, indicating new pathways for regulation.
Key Points
- The aim was to investigate how growth factors influence glucose transporter gene expression in fibroblast cell lines.
- Used fibroblast cell lines (BALB/c3T3, NIH3T3, Rat-2) for gene expression analysis.
- Stimulated cells with 15% calf serum and measured GT mRNA levels via hybridization.
- Assessed gene transcription changes using nuclear run-on technique.
- GT mRNA levels increased 5-10 fold within 3-4 hours of serum stimulation (p<0.01).
- Protein synthesis inhibitors did not block GT mRNA accumulation, indicating a direct transcriptional effect.
- Both protein kinase C-dependent and independent pathways were identified in GT gene expression regulation.
Cite This Study
Hiraki et al. (1988) studied this question.