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Identify, clone, and characterize the cDNAs encoding gap junction proteins in human and rat liver tissues.

September 1, 1986The Journal of Cell BiologyOpen Access

Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.

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Identify, clone, and characterize the cDNAs encoding gap junction proteins in human and rat liver tissues.
Screened a human liver cDNA library using an extended 58-mer synthetic oligonucleotide probe.
Evaluated gene copy number via Southern blot analysis and confirmed protein size through in vitro translation.
Screened a rat liver cDNA library using the isolated human cDNA as a probe to identify homologous sequences.
Identified a 1,574-base human cDNA encoding a complete 32,022-D gap junction protein.
Demonstrated by Southern blot analysis that the gene exists as a single copy conserved across multiple organisms.
Isolated a 1,127-base rat liver clone displaying high sequence homology to human cDNA in the coding region but divergence in the 3'-untranslated region.

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Implication

Molecular cloning study reveals homologous single-copy gap junction protein cDNAs in human and rat liver, indicating evolutionary conservation across species.

Cite This Study

Kumar et al. (1986) studied this question.

synapsesocial.com/papers/6a65574a3caefa6066033841 https://doi.org/10.1083/jcb.103.3.767

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Implication

Molecular cloning study reveals homologous single-copy gap junction protein cDNAs in human and rat liver, indicating evolutionary conservation across species.

Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.

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Discussion

Implication

Cite This Study

Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.

Key Points

Discussion

Implication

Cite This Study

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