What is the clinical evidence from this study?

Study design: Observational. Intervention: Feeding. Primary outcome: Increment of adipose tissue LPL after feeding and correlation with post-heparin plasma LPL.

What question did this study set out to answer?

The study aims to develop an assay for recovering lipoprotein lipase activity from human adipose tissue and to examine its correlation with post-heparin plasma enzyme levels.

July 1, 1985AJP Endocrinology and Metabolism

Human adipose tissue lipoprotein lipase: changes with feeding and relation to postheparin plasma enzyme

View Full Paper

Ask AI

Bookmark

Key result

Adipose tissue lipoprotein lipase activity eluted at 4 and 37 degrees C correlated strongly with post-heparin plasma LPL (r=0.725 and 0.754) and increased by 34% after feeding.

Key Points

The study aims to develop an assay for recovering lipoprotein lipase activity from human adipose tissue and to examine its correlation with post-heparin plasma enzyme levels.
Assay procedure developed using three methods for lipoprotein lipase recovery from biopsy specimens of human adipose tissue.
Elution performed at 4 and 37 degrees C using a buffer containing heparin and serum, with and without detergent extraction.
Correlation of enzyme activities both directly from tissue and from post-heparin plasma analyzed in female subjects of varying body weights.
LPL activity eluted at 4 degrees C showed a strong correlation with post-heparin plasma LPL (r = 0.725).
LPL activity eluted at 37 degrees C also correlated strongly with plasma LPL levels (r = 0.754).
The increase in LPL activity after feeding was about twice as high for the eluted methods (34%) compared to detergent-extracted activity (19%).

Study Design

Type

Observational

Structured PICO

Population

Female subjects of various relative body weights evaluated for adipose tissue lipoprotein lipase activity and its relation to post-heparin plasma enzyme and feeding.

Exposure

Assay procedures to recover lipoprotein lipase (LPL) activity (elution at 4°C, elution at 37°C, detergent extraction) and feeding

Outcome

Lipoprotein lipase (LPL) activity recovery and correlation with post-heparin plasma enzymesurrogate

Different methods of extracting LPL from human adipose tissue correlate strongly with each other and with post-heparin plasma LPL activity, providing valid indices of physiologically active LPL.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

May improve LPL recovery from adipose biopsies; leaves open standardization for metabolic research.

Cite This Study

Iverius et al. (1985) reported an observational. Feeding was evaluated on Increment of adipose tissue LPL after feeding and correlation with post-heparin plasma LPL. Adipose tissue lipoprotein lipase activity eluted at 4 and 37 degrees C correlated strongly with post-heparin plasma LPL (r=0.725 and 0.754) and increased by 34% after feeding.

synapsesocial.com/papers/6a656b8787c6eacc7af0c824 https://doi.org/10.1152/ajpendo.1985.249.1.e107

View Full Paper

Ask AI

Bookmark

Human adipose tissue lipoprotein lipase: changes with feeding and relation to postheparin plasma enzyme

Key Points

Study Design

Structured PICO

Discussion

Implication

Cite This Study

Human adipose tissue lipoprotein lipase: changes with feeding and relation to postheparin plasma enzyme

Key Points

Study Design

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider