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This research investigates how the bacterial SMC protein MukB reshapes DNA, focusing on knotting and supercoiling.

DNA Reshaping by MukB RIGHT-HANDED KNOTTING, LEFT-HANDED SUPERCOILING

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Randomized trial shows MukB regulates DNA shaping in E. coli, suggesting a conserved mechanism in chromosomal dynamics.

This research investigates how the bacterial SMC protein MukB reshapes DNA, focusing on knotting and supercoiling.
Purification of MukB and use of type-2 topoisomerase for DNA interactions.
Analysis of DNA knotting patterns generated by MukB.
Investigation of MukB's activity in the presence or absence of ATP.
MukB introduces right-handed knots into DNA with type-2 topoisomerase, stabilizing these structures.
The net supercoiling generated by MukB was negative, contrasting with eukaryotic condensins.
MukB can exist in two distinct forms, one of which is inert in DNA binding.

Petrushenko et al. (2005) studied this question.

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