What question did this study set out to answer?

To identify the principal metabolic pathways utilized for glucose degradation and energy generation in Rhizobium japonicum ATCC 10324.

March 1, 1969Journal of BacteriologyOpen Access

Glucose Catabolism in Rhizobium japonicum

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Authors

BKBernard B. KeelePediatric Nephrology of Alabama PHPAT B. HAMILTONMcGill University GEGerald H. ElkanAgricultural Research Service

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Implication

Laboratory study demonstrates glucose catabolism through the Entner-Doudoroff pathway in Rhizobium japonicum, highlighting a specialized route for bacterial energy production.

Key Points

To identify the principal metabolic pathways utilized for glucose degradation and energy generation in Rhizobium japonicum ATCC 10324.
Conducted radiorespirometric experiments tracking 14CO2 evolution from specifically labeled glucose, pyruvate, acetate, glutamate, and succinate in resting and growing cells.
Assayed cell-free extracts for the presence of key enzymes involved in glycolysis, the Entner-Doudoroff pathway, and the pentose phosphate pathway.
Resting cells released 14CO2 from labeled glucose at rates of 59% (C-1), 51% (C-2), 45% (C-3), 59% (C-4), and 43% (C-6), indicating catabolism proceeds exclusively via the Entner-Doudoroff pathway.
Extracts contained hexokinase, glucose-6-phosphate dehydrogenase, transketolase, and enzymes converting 6-phosphogluconate to pyruvate, whereas 6-phosphogluconate dehydrogenase was not detected.
Oxidation patterns of pyruvate (C-1=78%, C-2=48%, C-3=37%) and acetate (C-1=73%, C-2=56%) confirmed the coordinated operation of the tricarboxylic acid cycle.

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Keele et al. (1969) studied this question.

synapsesocial.com/papers/6a657b636c944c3eb20bbfeb https://doi.org/10.1128/jb.97.3.1184-1191.1969

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Implication

Laboratory study demonstrates glucose catabolism through the Entner-Doudoroff pathway in Rhizobium japonicum, highlighting a specialized route for bacterial energy production.

Glucose Catabolism in Rhizobium japonicum

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Implication

Key Points

Cite This Study

Glucose Catabolism in Rhizobium japonicum

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Implication

Key Points

Cite This Study

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