Asthma and chronic bronchitis were associated with a lower sputum MMP-9/TIMP-1 molar ratio compared to controls, which positively correlated with FEV1 values.
Observational (n=57)
Is airflow limitation in asthma and chronic bronchitis associated with an imbalance between MMP-9 and TIMP-1?
Airway inflammation in asthma and chronic bronchitis is associated with an imbalance between MMP-9 and TIMP-1, which correlates with airflow obstruction.
Asthma and chronic bronchitis are inflammatory diseases with extracellular matrix (ECM) remodeling and collagen deposition. Collagen homeostasis is controlled by metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). We evaluated MMP and TIMP balance in induced sputum of 10 control, 31 untreated asthmatic, and 16 chronic bronchitic subjects. We first performed zymographic analysis to identify the profile of MMPs. Zymography revealed a similar MMPs profile in all populations studied and that MMP-9 was the major enzyme released. We then measured, using enzyme immunoassay, the concentrations of MMP-9 and of its inhibitor TIMP-1 and evaluated whether airflow limitation may be associated with an imbalance between these enzymes. MMP-9 and TIMP-1 concentrations were greater in sputum of patients with asthma and chronic bronchitis than in control subjects. The molar ratio between MMP-9 and TIMP-1 was lower in asthmatics and chronic bronchitics than in control subjects, and positively correlated with FEV1 values. In asthma, MMP-9 levels were significantly correlated with the number of macrophages and neutrophils. This study shows that airway inflammation in asthma and chronic bronchitis is associated with an imbalance between MMP-9 and TIMP-1 which may have a role in the pathogenesis of ECM remodeling and airflow obstruction.
VIGNOLA et al. (Tue,) conducted a observational in Asthma and chronic bronchitis (n=57). Asthma and chronic bronchitis vs. Control subjects was evaluated on MMP-9 and TIMP-1 concentrations and their molar ratio in induced sputum. Asthma and chronic bronchitis were associated with a lower sputum MMP-9/TIMP-1 molar ratio compared to controls, which positively correlated with FEV1 values.