Key result
Overexpression of influenza polymerase polypeptides, specifically the viral PB2 protein, can titrate out the inhibitory action of the murine Mx1 protein, suggesting PB2 is its antiviral target.
The viral PB2 protein is suggested to be an antiviral target for the murine Mx1 polypeptide, as its overexpression titrates out Mx1's inhibitory action.
Mx1 may target viral polymerase; leaves open precise mechanism and therapeutic translation.
The murine Mx1 protein is an interferon-inducible protein which confers selective resistance to influenza virus infection both in vitro and in vivo. The precise mechanism by which the murine Mx1 specifically inhibits replication of influenza virus is not known. Previously, sensitive replication systems for influenza virus ribonucleoprotein, in which a synthetic influenza virus-like ribonucleoprotein is replicated and transcribed by influenza virus proteins provided in trans, have been developed. With these systems, the antiviral activity of the murine Mx1 protein was examined. It was found that continued expression of influenza polymerase polypeptides via vaccinia virus vectors can titrate out the inhibitory action of the murine Mx1 protein. This titration of inhibitory activity also occurs when the viral PB2 protein alone is overexpressed, suggesting that an antiviral target for the murine Mx1 polypeptide is the viral PB2 protein.
No takes yet. Share an insight, caveat, or question.
Huang et al. (1992) studied Influenza virus infection. Overexpression of influenza polymerase polypeptides (PB2 protein) was evaluated on Inhibition of influenza virus replication by murine Mx1 protein. Overexpression of influenza polymerase polypeptides, specifically the viral PB2 protein, can titrate out the inhibitory action of the murine Mx1 protein, suggesting PB2 is its antiviral target.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: