Key result
Cholecalciferol treatment in healthy rats moderately attenuated fatty acid metabolism and reduced the expression of L-type calcium channels and ryanodine receptors.
Why the study?
Given that cholecalciferol improves cardiac insulin signaling and glucose metabolism and reduces circulating non-esterified fatty acids, its effects on myocardial fatty acid metabolism and calcium handling were examined.
Does cholecalciferol modulate fatty acid metabolism and calcium homeostasis in the heart of healthy rats?
Does cholecalciferol modulate fatty acid metabolism and calcium homeostasis in the heart of healthy rats?
In a healthy rat model, cholecalciferol moderately attenuates fatty acid metabolism and fine-tunes calcium handling by reducing cytosolic calcium levels in the heart.
Cholecalciferol's cardiac metabolic and calcium effects in healthy rats warrant no practice change; leaves open relevance to human disease.
Purpose Besides the traditional role in regulating calcium metabolism, vitamin D regulates energy metabolism and heart function. Based on the data that cholecalciferol improved insulin signaling and glucose metabolism in the heart and reduced circulating non-esterified fatty acids, we examined the effect of this vitamin on the metabolism of fatty acids in the heart and the consequences on calcium handling responsible for regulating myocardial contraction-relaxation. Methods Twelve-week-old rats (n = 10 per group) were treated with cholecalciferol for six weeks. Blood lipid profile was determined. Western blot and qRT-PCR were used to examine protein and mRNA expression. Results Cholecalciferol did not affect the expression of proteins involved in the transport of fatty acids in plasma membranes and mitochondria of heart cells, the expression of the β-oxidation enzyme, acyl-CoA dehydrogenase long chain, and the level of peroxisome proliferator-activated receptor alfa and Lipin1 in nuclear extract of heart cells. However, cholecalciferol-treated rats had increased acetyl CoA carboxylase 2 protein expression and decreased expression of malonyl CoA decarboxylase that regulates carnitine palmitoyltransferase–1 function via malonyl-coenzyme A. In addition, the expression of uncoupling protein 3 was elevated. Also, the level of peroxisome proliferator-activated receptor-gamma coactivator in the nucleus of heart cells was increased along with the level of sarcoplasmic/endoplasmic reticulum Ca 2+ ATPase in microsomal fraction. In parallel, the expression of L-type calcium channel and ryanodine receptor was reduced. Conclusion In the heart of healthy rats, cholecalciferol moderately attenuates fatty acid metabolism and fine-tunes calcium handling in terms of reducing calcium levels in the cytosol.
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Ivković et al. (2022) studied Healthy (n=20). Cholecalciferol vs. Vehicle (corn oil) was evaluated on Expression of proteins and mRNA involved in fatty acid metabolism and calcium handling. Cholecalciferol treatment in healthy rats moderately attenuated fatty acid metabolism and reduced the expression of L-type calcium channels and ryanodine receptors.
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